Abstarct: Pectate lyase is one of the pectinases that catalyse the elimination reaction of α-(1→4) glycosidic bond in polygalacturonic acid and produce unsaturated oligogalacturonate. The presence of this enzyme has been demonstrated in many plants, fruits and microorganisms like bacteria and fungi. Since pectate lyase has important roles in biological processes like plant growth and development and fruit maturation, Pectate lyase is a key enzyme in pectate degradation in middle lamella. Moreover the presence of pectate lyase has demonstrated in many plant pathogens and facilitating their penetration. Here the gene that encoded pectate lyase from Cohnella A01 was isolated and cloned in pET26-b exxpression vector and expressed in E. coli BL21(DE3). The purification of the recombinant protein was performed. Biochemical properties of purified enzyme was assayed. The enzyme had an optimum activity at pH = 7 and 60 ºC. The activity of the enzyme was further demonstrated via bioassay and plate test. With these properties we propose that the enzyme has application in pectic wast water treatment, textile industry and oil extraction process.