Abstarct: Orientation to use unsaturated fatty acids in various fields as well as its importance in health, the cause to search for other sources of these fatty acids. In this present research, after extraction of total RNA and synthesis cDNA from Mortierella alpina, PCR amplification has been done using gene specific primers contents cozack sequence. The amplified fragment was cloned into the vector pBluescxript KS (+) containing the promoter napin. Then the recombination vector was transformed into E.coli DH5a. After confirmation of the positive recombinants using specific primers and Enzyme digestion (HindIII- SacI), designed recombinant vector Containing was cloned into the binary vector pBI121 by freezing and thawing Method, and transfered to Agrobacterium tumefacience LBA4404. Sequenced analyses showed The cloned CDS include 1374 nucleotides coding for 457 amino acids. Δ6Ds contain the amino-terminal cytochrome b5 domain carrying motif His-Pro-Gly-Gly (HPGG). Δ6Ds also have two membrane-spanning domains and three His-box motifs that are characteristic of membrane-anchored desaturases. Tools such as TMHMM, ProtParam and TargetP were used to predict the protein properties such as molecular mass, pI, signal peptide, transmembrane and conserved domains, secondary and spatial structures. then recombinant vector was used to integrate into the disc leaves genomic of tobacco plant. The transgenic plants were selected under selector condition which cotents 100 mg/l of kanamycin. The presence of delta-6 Desaturase gene was confirmed in the genome plants using PCR Techniques. RNA extraction and cDNA synthesis was performed of leaves tobacco. Loss of exxpression of delta-6 Desaturase gene was confirmed the proper functioning of the napin promoter and lack of exxpression in nonspecific tissues.