Abstarct: In the past, Human industrial and agricultural activity has led to increase in greenhouse gases emission, global warming and climate change in different regions of the world. The greatest and most important of which is carbon dioxide. To maintain continuous and storage of carbon in the soil is said carbon sequestration. Research has shown that control of nitrogen and carbon sequestration in the soil are the most effective ways to reduce greenhouse gas emissions to the atmosphere. In order to evaluate the effect of symbiotic microorganisms and fertilization on carbon sequestration, application of symbiotic microorganisms (non-inoculated, Bradyrhizobium, mycorrhizal, Bradyrhizobium + mycorrhizal) and external inputs of fertilizer (none-use of fertilizers, manure and Nchemical) a field experiment was conducted. The experiment was performed as factorial baxsed on the randomized complete block design with three replications. The results showed that the main effect of symbiotic microorganisms (bio-fertilizers) on the dry weight of stems and leaves, the distribution of carbon sequestration in the leaves and stem, leaf area, chlorophyll, increase soil organic carbon and soil respiration significantly increased and soil nitrate was significantly reduced by application of bio-fertilizers. Application of external inputs (fertilizer) on the distribution of carbon sequestration in the leaves and stem, leaf area, chlorophyll, total nitrogen, soil organic carbon, soil carbon sequestration and soil respiration significantly increased. The combined effects of symbiotic microorganisms and fertilizers used on any of the above traits was not significant. Among fertilizers, manure increased more carbon sequestration. The results confirmed that the application of manure and biological have effective role on sequestration and distribution of carbon on soybean and can prevent the release of greenhouse gases into the atmosphere.