Abstarct: Misconception of using more fertilizer, results in more yield is more common among the majority of Iranian farmers in one hand, and not using different types of organic and biological fertilizers on the other side, caused imbalances in nutrient of the soil and plant, soil degradation and environmental pollution. Considering that in Iran most arable lands are in calcareous beds and often they have pH over 8, because of that absorption of nutrient will make problem. One of the best ways to reduce soil pH, use of elemental sulfur and sulfur-oxidizing bacteria as acid producing agents. For this purpose, an experiment was designed entixtled Effects of sulfur granular, organic fertilizer vermicompost and Thiobacillus bacteria on red bean yield and some soil physical and chemical properties which was located in the city of Damghan Roudbar Chelated baxsed on factorial randomized complete block design with three replication and 36 experimental plots. Sulfur granular at three levels S0 = control, S1 = 500 kg ha-1, S2 = 1000 kg ha-1 application, vermicompost at two levels V0= control, V1= 5 tons ha-1 and Thiobacillus at two leveles T0= control and T1= applied. Analysis of variance of the data showed that the combination two factors of granulated sulfur and Thiobacillus bacteria causing significant increases in bean seed protein content and yield of protein and with increasing sulfur granulation levels, chlorophyll percentage and total sulfur was increase too. Use of 5 ton ha-1 of vermicompost could increase organic carbon and saturation percent. The main effects of treatments and their interactions effect could reduce soil pH, and therefore, according to the high lime in the soil, the lime content of the soil did not show significant decreases. In brief, we can conclude that use of granular sulfur with vermicompost and Thiobacillus bacteria can improve some chemical and physical properties of soil, but due to the low value of applied treatments it did not have significant effect on yield increase of bean.