Abstarct: Enthemopathogenic fungi such as Beauveria spp. have shown to be effective in controlling insect population. Beauveria spp., in addition of having a saprophytic life cycle on insect and plant remainders, have diverse host range, including but not limited to insects and spiders. According to differences in epi- and pro- cuticle composition, texture and structure, different suites of genes in fungi might be turned on with different exxpression patterns. These changes can be highlighted by either whole transcxript analysis or analyzing candidate transcxripts of fungal genes, facing varieties of insect cuticles. Here, two fungal Beauveria isolates with different strength of pathogenicities, weak and strong, were used. Cuticles of four insects, namely Tribolium castaneum, Galleria mellonella, Calliptamus italicus and Eurygaster integriceps were isolated and included within the fungal growth culture. Hence, four induced medium next to a minimal medium lacking a cuticle extract as check were used to grow two fungal isolates. Candidate genes, namely protease bassiasin, dipeptidyl peptidase, calcium sensor, cytochrome P450 and protein kinase were used to design gene-specific primers and study the transcxript changes via semi-quantitative RT-PCR. The first three genes were induced significantly facing cuticle containing media with highest exxpression value observed in C. italicus. In contrast and interestingly, down-regulation was observed for the two latter genes in induced media once comparing to check. Moreover, fungal isolates behaved differently in different media.