Abstarct: Abarkooh alluvial aquifer is located in southwest of Yazd city at the center of Iran. Groundwater is the main source for irrigation and domestic uses, considered as the potential causes of groundwater contamination in this plain. The study was carried out to assess hydrogeochemical properties of groundwater with the aim to find probable sources of major contaminants including nitrate and heavy mextals. In this regard 37 water samples were collected and analyzed for major ions (Ca, Mg, Na, K, Cl, HCO3 and SO4), nitrate and heavy mextals with environmental priority including arsenic (As), lead (Pb) and cadmium (Cd). Values of electrical conductivity (EC) increase from recharge area at the west to the eastern discharging parts where the playa is present. The abundance of cations and anions vary in the order of Na⁺>>Mg²⁺>Ca²⁺>Ka⁺ and Cl⁻>>SO4²⁻>HCO3⁻, respectively. Due to spatial variations of electrical conductivity and its relationship with the main ions concentration, the samples were divided into two groups: EC≤2050 μs/cm and EC> 2050 μs/cm. The highest values of Na and Cl are observed at the east where the saline water intrusion is probable due to the intensive abstraction of groundwater which reverses the flow direction. There is no dominant water type in the Abarkooh aquifer. Pb and Cd is highly correlated with Na, Cl and EC, confirm their geogenic source due to saline water intrusion. Nitrate is high at the west where the un-managed application of fertilizers in agricultural lands is so dominant. A geogenic source for As was proposed baxsed on the results of principal component analysis (PCA). The study confirms the main role of anthropogenic processes in contamination of Abarkooh aquifer generally resulted from over-exploitation of the aquifer and agricultural activities.