Abstarct: In this study a simple and sensitive directly suspended droplet microextraction (DSDME) method for preconcentation and determination Cu(II) by flame atomic absorption spectrometry was proposed. The analytical procedure baxsed on the formation of a complex between Cu(II) with 1- Phenyl- 1,2- propandione- 2- oxime- thiosemicarbazone (PPDOT) as a complexing agent. After extraction of the complex by octanol, copper concentration in solvent drop was determinated by flame atomic absorption spectrometry. The effect of different parameters such as pH, concentration of ligand, kind of buffer, kind and volume of organic solvent, volume of aqueous phase, extraction time, stirring rate of sample solution, temperature and ionic strength were investigated. Also, the effects of foreign ions were investigated. Under the optimized chemical and instrumental conditions, a linear calibration curve in the range of 0.0050-0.26 mg.L-1, with detection limit of 2.8 µgL-1 and enrichment factor of 25.6 were achieved. The relative standard deviations for six replicate determinations of 0.015, 0.030 and 0.22 mg.L-1 of copper(II) were %6.45, %3.45 and %3.74, respectively. This method was successfully applied for determination of copper(II) in the spiked water samples.