Abstarct: Nanoparticles and porous substrates have become very important in recent decades due to their high efficiency in adsorption of various types of gases, biomolecules and proteins. This fact led us to study the efficiency of a type of mextal organic frxame as a bed for Luciferase enzyme. This enzyme is one of the most widely used and almost the most important type of porous nanoparticles today, which is highly applied in medicine and biotechnology, but very sensitive to environmental changes. This study consists of three main parts: the first part, which includes enzyme stabilization on Fe-MIL-88(NH2) and Fe-MIL-88(NH2), coupled with Terephthalaldehyde, and the laboratory and device studies related to them, the second part consists of the enzyme stabilization on MIL-53 (Al) and its related device and laboratory studies, and the third part includes examination of applications including antibacterial, anti-oxidant, and anti-carcinogenicity properties of synthesized substrates. In summary, the results of this study indicate that binding of luciferase enzyme to both Fe-MIL-88 (NH2) and Fe-MIL-88 (NH2) coupled with Terephthalaldehyde is through a covalent bond and to MIL -53 (Al) is through adsorption. Studies also show that the stabilized luciferase on both mextal organic frxames has a higher kinetic and thermodynamic stability. Antibacterial, antioxidant, and anti-cancer properties of these compounds also indicate that both of them had a stronger antibiotic profile than Tetracycline and Kanamycin. These compounds have a significant antioxidant activity, and are cytotoxic with respect to MDA -MB-468, Hela and K562 cell lines.