Abstarct: In this study, a simple and reliable method is proposed for rapid and selective extraction and determination of the trace levels of Pd2+ ions by dispersive liquid-liquid microextraction (DLLME and flame atomic absorption spectrometry (FAAS) detection. In this approach, 2-mercaptobenzothiayole (2-MBT) was used as a chelating agent that forms a stable and hydrophobic complex with palladium(II) ions. Ethanol and carbon tetrachloride were selected as the disperser and extraction solvents, respectively. The influence of parameters such as pH, type and volume of buffer, type and volume of the extraction solvent, type and volume of the disperser solvent, ligand concentration, extraction time, centrifugation time and ionic strength were investigated and optimized. The effect of interfering ions were also investigated. Under the optimized conditions, Two linear calibration curve was achieved in the range of 0.020-1.20 mgL-1, with a detection limit of 0.0025 mgL-1 and enrichment factor of 35. The relative standard deviations for six replicate determinations of 0.040, 0.050, 0.10, o.40 and 0.10 mgL-1 of palladium(II) were 2.53, 3.01, 1.60, 1.10 and 0.47%, respectively. This method was successfully applied for determination of palladium(II) ions in water, soil and plant samples.