Abstarct: Abstract In this study, the dependence of the nucleon structure functions and the distribution functions of the nucleon-forming partons to the changes in the momentum transfer in the electron-nucleon deep inelastic scattering at different energies is investigated. To achieve this goal, it is necessary to calculate the Parton Distribution Functions (PDFs) in different energies. To calculate the distribution functions of the up and down valence quarks, we have used the theory of the parton model and the correlation of the structure function with the distribution functions of the quarks that make up the nucleon. In this study, we have used the information extracted in scatterings, so that a large number of scattering data of hard processes obtained in different high-energy laboratories in the transfer momentum Q^2=0.5-35GeV^2 and also for Bjorken's x_B range 0<x_B<1 to extract the proton and deuteron structure functions. Then, by applying the concepts of QCD theory and also examining nuclear corrections such as EMC effects and shadowing effects, to extract the neutron structure function from the structure function of existing nuclei, as well as examining Gottfried Sum Rule, which needs to be examined to calculate more accurate distribution functions. We have calculated the parton distribution functions in different ways by directly examining the data for different energies. In the following, with the physical analysis of the data obtained from the calculations and using the optimization method, we have presented a suitable model for the dependence of The Generalized Parton Distributions (GPDs) on the transition momentum. Then, using the presented model, we have calculated the Pauli and Dirac form factors and the electrical and magnetic from factors of protons and neutrons. Finally, we have compared the curves obtained in this study with the curves obtained using other models presented for GPD, and this comparison has been made with experimental data as well as lattice QCD. So that the improvement of the results obtained from the presented model and the impact of the effects considered in this research are evident for the nucleon form factors.