Abstarct: Pulsed laser deposition is a stoichiometric phenomenon which means that the plasma column formed on the target material has the same composition as the desired material, which reaches the surface of the substrate as a thin film. In any case, for using this technique correctly, it is necessary to know and check the parameters related to that. Among different calcium phosphates, Hydroxyapatite with the chemical formula Ca10 (PO4)6 (OH)2 has attracted a lot of attention due to its crystallinity and composition similarity to bone minerals. In this composition, the ratio of calcium to phosphorus is 1.67, which determines the activity of calcium-phosphorus compounds in the human body. In recent years, Hydroxyapatite has been intensively studied as a bone replacement material due to its specific biological and chemical properties. Its characteristics are completely consistent with the chemical composition of the mineral part of bones and teeth and it is used in repair and reconstruction surgery, implant coating, bone substitutes and implant composite components. The crystallinity of the ceramic coating is directly related to the solubility of the coating in the physiological environment and is an important factor in bioactivity. In this thesis, the growth and characterization of pure Hydroxyapatite thin films doped with different mextal ions on steel substrate was done. The deposition process is done using the second harmonic of the Nd:YAG laser in a vacuum chamber. Using X-ray and Raman diffraction analyses, laser pulse with energy 120 mj and frequency 8 Hz and annealing temperature 480° c were considered as optimal conditions for having a thin crystalline laxyer. With the help of field emission electron microscope analysis, we saw homogeneous laxyers with an irregular structure. Irregular morphology and crystal structure lead to surface expansion and increase in cell proliferation in medical applications. To improve the antibacterial properties of the thin laxyer, we used Hydroxyapatite doping with Copper and Zinc ions. Doping ions in pure Hydroxyapatite increases the contact angle and thus decreases the surface energy. On the other hand, with a decrease in surface energy and an increase in hydrophobicity, the adhesion of bacteria to the implant surface and the growth of hydroxyapatite laxyers in the body simulation solution are reduced. In this research, the electron temperature and density of the plasma produced by laser pulse on the hydroxyapatite target material were investigated by optical spectroscopy, baxsed on the change of laser parameters such as energy and frequency.