Abstarct: In this research, surface morphology, structural, optical, electrical and thermoelectrical properties of vanadium oxide samples prepared by spray pyrolysis technique were investigated. In order to characterize the samples, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Raman spectroscopy, UV-Vis optical spectrometry, flow-voltage characteristic and the Seebeck effect device have used. The specimens were prepared on glass substrates by spray pyrolysis technique. In this research, the spray precursor was vanadium (III) trichloride (99.9%). The variable parameters in this research are as following: precursor concentration (section 4-2), solution deposition rate (section 4-3), nozzle to substrate distance (section 4-4), annealing samples in various atmosphers (section 4-5) and substrate temperatures (section 4-6). The FESEM images of synthesized samples indicated the formation of nano-grains less than 100 nm in size. According to EDX spectra all samples suffers from oxygen vacancies in their crystalline lattice. The X-ray diffraction patterns indicating that in most cases the synthesized laxyers have a polycrystalline orthorhombic structure in V2O5 phase with preferred direction (001) and in some cases with additional phase of V3O7. The peaks of Raman spectra also confirmed the formation of V2O5 phase. I-V characteristic showed that the prepared samples have a relatively high sheet resistance of about 100 KΩ. Finally, the Seebeck effect confirmed that all the synthesized samples have n-type conductivity.