Abstarct: In recent years, blockchain as emerging information technology has transformed the software development industry. Therefore, many organizations have adopted blockchain-baxsed technology in software development at different organizational levels. However, the blockchain implementation at the organization levels can be associated with several technical, organizational, legal, financial, and environmental challenges which consist of intra-organizational and inter-organizational. Analysis of these challenges will play a significant role in organizational performance. This study has been investigating the challenges of blockchain implementation for the software development industry in Iran. baxsed on the experts’ opinions, challenges identification has been conducted via the two-phase fuzzy Delphi method for medium and large-sized organizations. Extensive quantitative and qualitative obtained challenges are evaluated via the best-worst fuzzy method. Obtained results indicate that smart contracts vulnerabilities and deterministic execution of the technical challenge group have the most critical importance and governance of the legal challenge group has the least critical importance. It is mentionable that most technical challenges have higher priority than other considered challenges.