Abstarct: Objectives: the aim of the present study was to investigate the effect of beta-alanine supplementation on aerobic and anaerobic capacity and responses of blood lactate and some indices of muscle damage to a single bout of exhaustive exercise in female basketball plaxyers. Methods: In a semi-experimental study, 18 female basketball plaxyers (Age: 22.77±1.92 years, weight: 60.87±5.27 kg, height: 167.56±5.11 cm, body mass index: 24.35±1.06 kg/m2) baxsed on the estimated VO2max were assigned into experimental (N=9) or control (N=9) groups. Prior to and after preparation period (lasting for 4 weeks) bruce test was applied as the exhaustive. Blood samples were taken before and following the exercise test in both pre and post-tests to be analyzed for blood levels of lactate and serum levels of lactate dehydrogenase and creatine kinase. During the preparation period the subjects received 6.4 gr/day beta-alanine supplement or starch as placebo. Data were analyzed using SPSS22 software. Normal distribution of the data were assessed using Shapiro-wilk normality test and data were analyzed using repeated measure analysis and paired t-tests at significance level of P<0.05. Results: The results of the present study indicated that beta-alanine supplementation along with preparative training had no significant effect on aerobic and anerobic capacity assessed by Bruce and Wingate tests (P>0.05). Moreover, we observed that serum levels of CK and LDH increased significantly in response to exhaustive exercise and beta-alanine supplementation has no significant effect on this response in female basketball plaxyers (P>0.05). However, beta-alanine supplementation attenuated blood lactate respons to exhaustve exercise (P<0.05). Conclusion: baxsed on the findings of the present study we conclude that although beta-alanine had no significant effect on exercise performance, it improved buffering capacity and may delay exercise-induced fatigue. Moreover, we concluded that beta-alanine may have no ani-oxidative potential but due to the limited researches it needs to be explored in the future studies.