Abstarct: Resistance training has an important role in reducing the symptoms of menopause and hormonal changes (1) and may be the combination of resistance training and low-calorie diet affect these changes, the aim of this study is to evaluate the effects of resistance training and low calorie diet on relaxin serum levels of postmenopausal women, after calling 20 post-menopausal women were purposefully selected and randomly divided into two groups (experimental group and control group, n = 11 n = 9), the experimental group exercise for 8 weeks and every week 2 days with the intensity of 20-30% 1RM, the control group continued their activities during a period of 2 months, relaxin serum levels in the fasting state, in two stages, one week before the start of the training program and 48 hours after the last training session in the eighth weeks was carried out, body composition with In body device before and after the training period was measured, the covariance and Pearson correlation test analysis were used for data analysis, level of significance was considered )p≤0/05. The results showed that eight weeks of resistance training and low-calorie diet significantly reduced total cholesterol levels, triglycerides, LDL, relaxin and an increase in HDL values before the intervention, also body weight, fat mass, fat percent, lean body mass and BMI of the experimental group was significantly decreased, while the decrease of related indicators of abdominal obesity (waist size, hip and WHR) statistically is not significant, and also there were no significant relationship between relaxin serum levels and the components of body composition, and the relationship between relaxin serum levels and blood lipids in cholesterol serum level and LDL was significant, it seems that decreased relaxin serum levels induced by caloric restriction combined with resistance training can be effective in reducing the symptoms of menopause and affect them, but due to the lack of history or research literature, for getting best and more accurate results in this field more researches are needed,