Abstarct: Background and Aim: One of the dangers that has been raised in recent years is vitamin D deficiency, which is associated with many cardiovascular disorders. Arterial stiffness responses also depend on nutritional and exercise factors. Therefore, the aim of the present study was to investigate the relationship between arterial stiffness response after glucose uptake and exercise with baxseline levels of vitamin D in inactive women. Methodology: The present study was a quasi-experimental study and the subjects of the present study were 43 inactive women in Shahroud with an age range of 20 to 42 years. All subjects were present in the physical education laboratory of Shahroud University of Technology on an empty stomach and vitamin D levels were measured; Arterial stiffness and baxseline blood glucose levels were also measured. Subjects then worked for 65 minutes at 65% of maximum heart rate, after which blood glucose levels were measured immediately. Also, 75 g of glucose was given to the subjects after 30 minutes of activity, 30 minutes after the end of glucose consumption (recovery stage), arterial stiffness was measured by Vascular Screening device. Data were analyzed using SPSS software version 21 and Pearson correlation test and analysis of variance with repeated measures at a significant level (P ≤0.05). Results: The results showed that there was a significant relationship between changes in CAVI and vitamin D (P = 0.001); But changes in ABI with vitamin D were not significant (P = 0.414) .There was also a significant and inverse relationship between baxseline index (CAVI) and vitamin D in inactive women (P = 0.001); But there was no significant relationship between basal ABI and vitamin D in inactive women (P = 872). ConclusionAccording to the results, the response of arterial stiffness (CAVI) to vitamin D levels was significant and inverse. This indicates the effect of vitamin D on CAVI changes. The lower the level of basal vitamin D, the greater the stiffness of the arteries and vice versa. Since the response of other arterial stiffness (ABI) indicators was not related to baxseline vitamin D levels, more research is needed.