Abstarct: Oxidative stress plays a key role in the pathogenesis of type 2 diabetes (T2D) and its complications. Antioxidant supplements and exercise are two potential approaches to delay the development of T2D. Therefore, the purpose of this study was to evaluate the effect of 4 weeks high intensity interval training (HIIT) with supplementation of spirulina on serum levels of MDA and TAC in inactive Type 2 diabetes patients. This quasi-experimental study included pre-test and post-test, with 3 experimental groups and 1 control group. The statistical sample of this study was 55 women with type 2 diabetes, with a mean age (51.95 ± 5. 57 years) and (BMI 30.45 ± 3.63 kg / m2) who were randomly divided into 4 groups: 4 Group 1: HIIT exercises with spirulina supplementation (15 subjects); 2: HIIT exercises (15 subjects); 3: spirulina supplementation (15 subjects); 4: control (10 non-exercised and supplemented). (Matching baxsed on Blood glucose and BMI of the subjects). Groups 1 and 3 supplementation groups was daily consumed 2 g of Spirulna. Blood samples were collected before exercise and 48 hours after completion of exercise and consumed of Spirulna in Fasting. Training sessions consisted of 45 minutes walk or running on a treadmill. Each session was warmed up to 10 minutes with a 50 to 70% HRR intensity of 10 minutes, with a 50 to 70% HRR intensity and 25 minutes of HIIT (training sessions were divided into four sections for 4 minutes, 85-95% of HRR with 3-minute rest periods, 50-70% HRR intensity) was divided. The t-test was used to assess the difference in the group, ANOVA test was used to assess the difference between the groups and the Bonferroni post hoc test to determine whether the difference was from which group. All evaluations were performed with SPSS software at a significant level (P≤0.05). The findings showed that the MDA level was significantly decreased in all three groups of exercise + supplementation (P = 0.03), exercise (P = 0.001) and supplementation (P = 0.013). Also, the serum level of MDA in the groups (P = 0.03) showed a significant difference. According to the Bonferroni test, this difference was due to the difference between the Training group and Supplemention group with control group. Other results showed that changes in serum TAC level (P = 0.745) were not significant. According to these results, HIIT exercises and spirulina supplementation are a good stimulus to reduce the level of harm caused by free radicals and consequently reduce risk factors in women with type 2 diabetes.