Abstarct: This is a quasi-experimental study with pre test-several post test design with done via the crossover method. The purpose of this study was to determine the effect of a one-session resistance practice with high and low intensity and with no blood flow restriction of trained students, on peptide natriuretic atrial serum. For this purpose, 8 men were chosen randomly among all the students at Kharazmi University, with ages in the range [25.75 ± 1.75] and fat percentage in the range [15.4 ± 7.7]. These men did different resistance exercise with rest intervals of 48 hours, then 3 cc of their blood was sampled and the levels of peptide natriuretic atrial serum were measured by Elisa kit. Data were analyzed by SPSS software version 22 and variance analysis with repetitive measurements. Results showed that the comparison of peptide natriuretic atrial serum levels in effect of imposing resistance exercises with different methods doesn’t have a significant difference with each other (P ≤ 0.05). But the comparing the means revealed that resistance exercise with high and low intensity under blood flow restriction causes into higher levels of peptide natriuretic atrial. Thus, exercise with blood flow restriction can probably be a safe and also efficient method for practicing in order to increase the peptide natriuretic atrial levels. However, practices with blood flow restriction with high intensity, paly a more prominent role.