Abstarct: An important part of environmental pollution is related to the effects of heavy mextals that factors such as erosion, volcanoes and springs, human activities such as the use of fossil fuels and agriculture and mining cause the accumulation of these mextals in soil and toxicity to plants, animals and Societies become human. In the present study, the effect of heavy mextal salts of iron, zinc and copper on homocyte abundance, nutritional index and biological characteristics of Mediterranean flour moth Ephestia kuehniella Zeller (Lep .: Pyralidae) in vitro (25 ± 3 ° C, 40 5 5 relative humidity The percentage and light period were 14:10 h (darkness: light). The fourth instar larvae of this storage pest were treated with iron, zinc and copper salts at concentrations of 25, 50 and 100 mg / kg in 24 and 48 hours. Immunological results showed that there was a significant difference in the total hemocyte count, plasmotocytes, granulocytes and the activity of phenol oxidase enzyme at the level of 1% probability with the control. Iron mextal salt at concentrations of 25 in both time periods and 50 mg / kg after 24 hours caused a decrease and at a concentration of 100 mg / kg after 24 and 48 hours caused a significant increase in the total hemocyte count compared to the control. Copper and zinc mextal salts at all concentrations increased the total hemocyte count after 24 and 48 hours compared to the control. Iron mextal salts at all concentrations after 24 and 48 hours, copper at 25 concentrations after 24 hours and zinc at 50 concentrations after 24 and 48 hours caused a significant decrease in plasmotocytes compared to the control. Iron at all concentrations and at all times showed the greatest effect on the increase of granulocytes compared to other treatments. In measuring the activity of phenol oxidase enzyme, iron salt and zinc at a concentration of 100 mg / kg at both 24 and 48 hours showed the highest effect on increasing the activity of the enzyme, while iron at a concentration of 50 mg / kg caused a significant decrease. The activity of this enzyme was increased after 24 hours compared to other treatments. In terms of nutritional indexes, copper mextal salt at a concentration of 25 mg / kg caused an increase in relative growth rate compared to the control. In evaluating the relative consumption rate index and Efficacy of Convertion of Ingested Food index, no significant difference was observed in any of the salt concentrations of iron, zinc and copper mextals compared to the control. Feeding Detterent Index was significantly different between all treatments. The salts of the above mextals affected the biological properties of the Mediterranean flour moth. So that copper and zinc at a concentration of 100 have the greatest effect on Larval duration , copper at a concentration of 100 has the greatest effect on pupal duration, copper at a concentration of 100 has the greatest effect on pupal weight loss and copper and zinc at a concentration of 100 has the greatest effect on Exit percent of adult. None of the mextals had a significant effect on Longevity adult. The present study showed that heavy mextals have a great impact on the biological and safety characteristics of Mediterranean flour moths, and understanding the impact of environmental factors and stresses on nutritional behavior and immune responses can be effective in adopting pest control programs.