Abstarct: The phenolic compounds have been known to be very toxic and carcinogenic for the human being. These contaminants can be observed in the effluent of most of industries such as petroleum refinery industries, chemical species production factories, explosive materials factories and cock industries; Also these are used in the production of disinfectants, artificial poisons (Resins), pesticides and oil. Nowadays, the use of Advanced Oxidation methods of purification of wastewaters from phenolic compounds is under the focus. The ozonation process, which is one of the methods of Advanced Oxidation, is very efficient for removal of organic compounds like phenol. Also the Biological methods of purification, such as the use of Activated Sludge and different Bacteria has been investigated in literature to be convenient for removing of organic compounds due to their low expenditure. In this project, the two more important approaches of removal of phenolic compounds, Ozonation process and biological purification, has been used. After some experimental work, it was observed that the performance of ozonation system for the concentration of 100, 200, 300 and 400 ppm was very high and also the maximum 99% phenol removal was reached for these concentration after 15,35, 55 and 75 minutes, respectively. Also the effect of pH of phenolic solutions was investigated the pH equal to 11 was observed as the optimal pH in the experiments. Also it was revealed that the Hydrogen Peroxide has not a remarkable effect on the reaction velocity and improving the removal of phenol. In this research, also the efficiency of the Activated Sludge, which is one of the most common approach of Biological methods for purification of wastewaters, was investigated and the effective parameters were optimized. The optimal pH for this process was investigated to be 7 and also 5ml insemination of Activated Sludge showed the best removal of phenol in the minimum contact time. Also by using of 100, 200, 300 and 400 ppm, the maximum removal of phenol was obtained after 18, 21, 24.25 and 28 hours, respectively.