S392 : Study on the effects of extracts of several botanical specieses and entomopathogenic fungi Beauveria bassiana Balsamo for controlling stored-product pest of Indian meal moth Plodia interpunctella Hubner in vitro
Thesis > Central Library of Shahrood University > Agricultural Engineering > MSc > 2017
Authors:
Jamileh kesalkheh [Author], Ali Darakhshan Shadmehri[Supervisor], [Advisor]
Abstarct: The insecticidal effects of extracts of four plant species including Melia azedarach L., Eucalyptus citriodora Hook , Nerium oleander L., Rosmarinus officinalis L. and three isolates of the entomopathogenic fungus Beauveria bassiana Balsamo were evaluated on third instar larvae of Plodia interpunctella Hubner at 26¬ ± 1 ° C , 70 ± 3% RH and 16:8 h photoperiod. Experiments were conducted using a completely randomized design (CRD) with four replications. Acetone was used as a control. The mortality of larvae, the number of larvae become pupae, the number of healthy adult insects and longevity of adult insects were recorded. Results of herbal extracts showed that 36 hours after treatment, oleander 90% and rosemary 80% with a mean mortality 90% and chinaberry 15% with 0% mortality had the highest and lowest mortaliy effect on Indian meal moth larvae, respectively. Rosemary had the lowest LC50 of 41.22% and chinaberry had the highest LC50 of 77.4% .In the experiment of 48 hours after treatment, eucalyptus 85% with mean of 92.5%, oleander 90% and rosemary 80% with mean of 90% had the highest, and chinaberry 15% with an average of 0% had the lowest effect on the mortality rate of the larvae. In this experiment rosemary had the lowest LC50 of 33.47% and chinaberry had the highest LC50 of 59.69%. The highest number of pupae observed in eucalyptus 15% treatment with mean of 92.5% and the lowest number of pupae was observed in eucalyptus 85% treatment with a mean of 0%. The highest number of adult insects was obtained from chinaberry 15% treatment with an average of 85% and the lowest adult insects was obtained from eucalyptus 85% treatments and rosemary 80% with a mean of 0%. Maximum insect lifespan observed in treatments oleander 30% and chinaberry 15% with an average of 6.5 days and rosemary 15% with an average of 6.25 days and the lowest of it observed in eucalyptus 85% and rosemary 80% with an average of 0%. The results of fungal isolates showed that the highest larvae mortality found in Bb3 isolates with an average of 82.5% and the lowest larvae mortality observed in Bb1 with an average of 45%. The highest number of pupae observed in Bb2 treatment with a mean of 42.5% and the lowest number of pupae observed in Bb3 treatment with an average of 17.5%. The highest number of healthy adult insects observed in Bb2 treatment with 32.5% and the lowest number in Bb3 treatment with a mean of 12.5%. The highest adult lifespan was observed in Bb1 treatment with an average of 4.25 days and the lowest in Bb3 treatment with an average of 3.25 days. The lowest LT50 in Bb3 treatment was 3.3 days and the highest in Bb1 treatment was 9.4 days.
Keywords:
#B. bassiana #plant extract #Indian meal moth #Nerium oleander #Eucalyptus citriodora #Rosmarinus officinalis #Melia azedarach Link
Keeping place: Central Library of Shahrood University
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