Abstarct: One of the potential of increasing rice production in Mazandaran can be the ability to replant rice after its cited first rice crop harvest. Therefore, in order to investigate the effect of nitrogen on growth, concentration of mineral elements and quantitative and qualitative yield of three rice varieties in replant, split plot experiment, in a randomized complete blocks design with three replications in was carreied out in the field of deputy of the Rice Research Institute rice of Mazandaran (Amol) in 2016. The treatments experiment to include of TaromHashemi, Binam and Koohsar rice varieties as the main factor and nitrogen fertilizer at four levels of 0, 30, 60 and 90N kg/ha of urea source as a subplot. The results showed that nitrogen fertilizer treatments had a significant effect on traits of the plant height, tiller number and growth indicators such as Leaf Area Index (LAI), Net Assimilation Rate (NAR) and chlorophyll content of leaf, which was measured at flowering stage. In the between of varieties, TaromHashemi status was better than Binam and Koohsar varieties. Nitrogen fertilizer treatments also had a significant effect on traits of the plant height, number of panicle, yield and grain yield components, as well as biological yield in the physiological maturity stage. At this stage, Koohsar cultivar was command more preference toward Binam and TaromHashemi varieties. Nitrogen concentration, protein percentage and qualitative traits such as amylose percentage and grain gelatinization temperature were affected by nitrogen, but concentration of phosphorus, potassium and trait gel of consistency grain was not affected by nitrogen. In the three varieties, TaromHashemi variety was better than the other two varieties in terms of nitrogen percentage, protein percentage, gel of consistency and grain gelatinization temperature. Rice yield improved with increasing nitrogen consumption. Therefore, for the Koohsar cultivar, 90 kg/ha of Net-Nitrogen is carried out three times in a 15-day interval, and for TaromHashemi and Binam varieties, 60 kg/ha of Net-Nitrogen is recommended for three times in 20 days’ intervals.