Abstarct: Menth arvensis L. is one of the most important medicinal plants from Lamiaceae family. Aerial parts of the plant contain a various number of aromatic compounds that used in food, cosmetic and pharmaceutical industries. Largescale production of Mentol has been essential target in recent years because of high commercial value of this plant. This study was carried out in two different phase. . In first phase, lateral meristem explants were disinfected and cultured on ½ MS (Murashig & skoog, 1962).Then, sterilized explants were cultured on MS, 1/2MS and ¼ MS supplemented with 0 or 2 mg/l activated charcoal and transferred to growth room with light intensity of 2500-4500 Lux for optimization of micropropagation.Leaves explants were cultured on MS media supplemented with 0, 0.5, 1 and 2 mg/l of 2,4- dichlorophenoxyacetic acid (2,4-D) or Naphthalene acetic acid (NAA) alone or in combination with 6-Benzylaminopurine (BAP) or Kinetin (KIN) in concentration of 0, 0.5, 1 mg/l for callus induction.To determine the best medium for the cell suspension phase, leaves and callus explants were cultured on N6, ER,WPM, MS and B5 media supplemented with 0.5 mg/l of 2,4-D.After optimization of appropriate callus culture medium, calli were cultured on MS or B5 media complemented with 0.5 mg/l of 2,4-D with various concenteration of activated charcoal, citric acid, ascorbic acid, turmeric powder and polyvinylpyrrolidone (PVP) for assess of biosynthesis change of phenolic compounds.In the second phase, calli were transferred to suspension MS and B5 media Supplemented with 2,4-D in concentration of 0, 0.25, 0.5 and 0.75 mg/l and 30 or 60 gr/l sucrose. After accessing to appropriate volume of callus biomass, the growth index was surveyed baxsed on dry weight and number of cell. After cell proliferation and reaching to the suitable growth phase, cell suspension were elicited with methyl jasmonate in concentration 1 and 2 µl for 48 and 72 h and 5 and 10 mg/l of chitosan for 72 and 96 h.The result indicated that ½ MS media complemented with 2 gr activated charcoal have the greatest impact (12.6 and 0.035 mm) on number and diameter of stem The highest amounts of fresh and dry weight of callus (12.3 and 1.88 gr) were observed on MS media supplemented with 0.5 mg/l of 2,4-D. In comparison between different media, the maximum dry weight (0.6 gr) was indicated on B5 medium. The greatest amount of phenol (127 ml/gµ) in media and also lowest quantity (72.9ml/gµ) of phenol in callus was observed in media with 1000 mg/l of turmeric powder.In suspension phase, maximum amount of dry weight was observed on B5 media with 30 and 60 gr/l of sucrose. A s well as, the dry weight significantly increased on 0.5 and 0.75 mg/l of 2,4-D. Moreover, both of maximum cell growth index (0.25 gr) and highest number of cell were indicated in B5 medium with 60 gr/l sucrose in sixteen days of growth. As well as, some important compounds such as p-cimine, dodecanol, tetra decanol, eugonol, were illustrated. The result of this study could applied as beneficial alternative for production of secondery mextabolites by biotechnological approch such as cell culture.