Abstarct: Nowadays, several factors are involved in the way organizations operate, therefore, identifying and using these factors plays a crucial role in the survival of organizations. The purpose of this research is to investigate the effect of reputational capital and ethical atmosphere of the organization on the relationship between organizational DNA and organizational performance. For this purpose, the required data from a sample of 320 employees of the Melli Bank Branches in Mashhad city were collected. Data gathering is applied via using standard questionnaires include Victor and Coleman’s ethical atmosphere, reputational capital of Fombrun & Van Rail, Organizational Performance of Hersey & Goldsmith, and made-researcher questionnaire organizational DNA. The validity of questionnaires baxsed on content validity method and reliability of the questionnaires baxsed on Cronbach's alpha method are assessed. Finally, a structural equation modeling is adopted to examine the relations between considered variables via using statistical analysis software, namely SMART PLS. The obtained results show that organizational DNA has a significant impact on the performance of the organization. In addition, prominent capital also mediates this relationship. The research findings also indicate that the organization’s ethical climate modifies the impact of organizational DNA on organization performance in the branches of the Melli Bank.