Abstarct: Tissue engineering is now a promising strategy to treat patients who have tissue damage using cells in combination with polymeric scaffolds. Several synthetic and natural biomaterials are proposed for fabricating tissue-engineering scaffolds. In the meantime. Polyvinyl alcohol as a bio-polymer is used in tissue engineering applications as a scaffold. In this study, polyvinyl alcohol-fibrin nanobiocomposite scaffold was developed for wound dressing application. The polyvinyl alcohol scaffolds were fabricated by electrospun method, and fibrin glue with different concentrations of the fibrinogen and thrombin wasinjected into the scafolds. The polyvinyl alcohol scaffolds have good mechanical propertie. In order to estimate microstructure properties SEM analysis was used. The fiber diameters were estimated about 300-400nm. In addition, the size of pores was 10-20 micron which were decreased with increasing fibrin concentractions. Contact angle test was used in order to estimate hydrophobic and hydrophibic properties. Results demonstrated that polyvinyl alcohol-fibrin nanocomposite have superior hydrophibic properties when compared to polyvinyl alcohol scaffold alone. This result is consistant with total cell numbers which was achieved by a flurescence microscope data. The cell proliferation was evaluated by MTT test on Mesenchymal Stromal Cells for 4 days. The MTT results of the cell culture experiments demonstrated that the cells were able to attach and proliferate on the polyvinyl alcohol-fibrin nanocamposite scaffolds. The results showed that PVA-fibrin nanocomposite scaffolds have the potential to be used in wound dressing tissue engineering.