Abstarct: Today, each of us and our loved ones are exposed to various forms of cancer, regardless of age and gender, due to lifestyle changes. In recent years, the use of electroporation process has attracted much attention, due to its application in various military, industrial and medical fields. Electroporation is a microbiology technique which creates tiny holes in the cell membrane by the applied electric field. The electroporation process needs high-voltage pulses to provide the required electric field. High-voltage pulse generators can be mainly divided into two major groups: Classical pulse generators and power electronics-baxsed pulse generators. As their name suggests, the first group is associated with the primary and elementary pulse generators like Marx generators, and the second group is associated with the pulse generators that have been updated with the advancement of power electronics like Modular Multilevel Converters. These two major groups are also divided into several subgroups which are reviewed in detail in this thesis. This thesis reviews and examines the literature presented in the field of pulsed power and pulse generators proper for the electroporation process and addresses their strengths and weaknesses. Several tables are provided to highlight and discuss the characteristics of each subgroup. A comparative study among different groups of pulse generators is performed. In this thesis, the aim is to design and simulate a hybrid pulse generator baxsed on a modular multilevel converter, which has the ability to produce pulses with amplitude in the range of several kilovolts and a pulse width in the range of several microseconds, which can be used for the electroporation process, especially in removing cancerous tumors are used. After design and mathematical analysis, the proposed topology was simulated in Matlab/Simulixnk environment. The simulation results confirm the performance of the proposed structure. Finally, conclusions have been drawn and suggestions for future research have been made. .