Abstarct: Steel plates are used in many structures, such as deck and bottom of ship structures, plate the box girders of bridges, platforms of offshore structures, and structures used in aerospace industries. In many occasions these plates are subjected to axial compressive forces, which makes them prone to instability or buckling. In many cases, it is almost inevitable to have holes in the plate elements for inspection, maintenance, and service purposes. In such cases, the presence of these holes redistributes the membrane stresses in the plates and may reduce their stability significantly. Therefore, it is necessary to carefully investigate the buckling behavior of these plates. In this investigation, the buckling behavior of rectangular perforated plates with different holes numbers, shapes, sizes and positons has been studied both numerically and experimentally. Different aspect ratios for plates are used to study effect of these parameters on buckling load. FEM using ABAQUS software is used for the numerical investigation. The finite element results have been agreed well the experimental results.