Abstarct: Scrobipalpa ocellatella Boyd (Lep: Gelechiidae), is one of the most important pests of sugar beet in Iran, Which annually causes irreparable damage to this country's economic product. The use of digestive enzymes inhibitors or digestive enzymes-inhibiting genes that are transmitted to the plant can be considered as a new strategy for controlling this pest. The first step in the implementation of these methods is to identify the digestive enzymes in the gastrointestinal tract of the beet larvae. In this study, the biochemical properties of glucosidase and protease in the gastrointestinal tract of the last larval period of the pest were studied. Scrobipalpa ocellatella were collected from the fields of jovein during the growing season. The digestive tract of insects was isolated and stored at -20 ° C. Enzymic activity and biochemical properties including pH, temperature and ion effects were measured using substrates and reagents of each of the enzymes mentioned. The optimal α and β-glucosidase temperatures were calculated at 40 ° C. The highest activity of α and β-glucosidase enzymes were investigated in the presence of specific substrates in different acidity and its optimum at pH 8 was obtained. Maximum activity of total protease acidity in the digestive tract of this pest was determined using the azocasin substrate and equal to pH = 11- 9. Optimum total protease temperature was measured at 35 ° C. The activity of specific proteins trypsin, kimotrypsin and elastase was investigated using special substrates in the range of acidity and their maximum activity was respectively, 10, 11 and 11. The optimal temperature for the three proteases mentioned was respectively 35, 40 and 40 ° C. The effect of mextallic Fe2+, Mg2+, Mn2+, Zn2+ and Cu2+ with concentrations of 5 and 10 mM, was significant for α and β-glucosidase enzymes, trypsin, chymotrypsin and elastase. In addition, increased 5 mM of Mg2+, 10 mM of Mn2+ and 5 mM of Cu2+ , reduced the activity of the α-glucosidase enzyme and 5 mM of Fe2+, which increased the activity of β-glucosidase enzyme. All of these ions increase the activity of trypsin and elastase enzymes.