S436 : The study of seed deterioration and hormone priming on enzyme changes and quantity & quality yield of corn (Zea maysL.)cultivars
Thesis > Central Library of Shahrood University > Agricultural Engineering > PhD > 2018
Authors:
Saedeh Rashidy [Author], Hamid Abbasdokht[Supervisor], Ahmad Gholami[Advisor], Reza Tavakolafshary [Advisor]
Abstarct: Seed deterioration is one of the weakening factors of seed viability and it is a limiting factor for its germination. In order to study seed deterioration and hormone priming on enzymatic variations and qualitative and quantitative performances of corn varieties, laboratory and farm researches were carried out for 2 years. Laboratory researches were performed at seed and biotechnology laboratory, department of agronomy and plant breeding, Faculty of agronomy and animal sciences, Tehran university (Karaj) in 2015. This experiment was a factorial test and was done in a completely randomized design with 4 replications. The studied varieties included; single cross 704 and single cross 260. At first, standard germination test was performed on corn varieties to determine the viability. Then the seeds were subjected to early aging test at 0, 5, 10 and 15 days in order to create different viabilities. After early aging test, the effects of 0,50, 100 and 150 µmol concentrations of cytokinin and gibberellin were investigated on deteriorated seeds of corn varieties. In all tests, germination percentage, germination rate, radicle length, fresh weight of radicle, fresh weight of plumule and dried weight of plumule were measured. The results showed that seed deterioration can lead to the reduction in all measured traits of corn varieties. The effect of early aging treatment was significant for the varieties. In single cross 704 variety, the measured traits for early aging treatment revealed lower reduction in comparison to single cross 260 variety. The treatment of deteriorated seeds with gibberellin and cytokinin hormones helped to the improvement of the measured traits. The effect of higher concentrations of hormones was significant in prolonged aging treatments. In addition, the results revealed the cytokinin hormone could improve germination traits more than gibberellin hormone. In the following, the final test was done in the laboratory in order to determine the enzymatic activity of antioxidant catalase and peroxidase in the corn varieties which were under early aging treatment and investigation of hormone priming effect (cytokinin and gibberellin) on the enzymatic activity of deteriorated seeds. The results showed that seed deterioration leads to the decrease in the activity of antioxidant peroxidase and catalase of corn varieties. The treatment of deteriorated seeds with cytokinin and gibberellin hormones increased the activity of antioxidant enzymes in deteriorated seeds of corn varieties. Cytokinin hormone had more effects on the increase of antioxidant enzymes activity in deteriorated seeds comparing to gibberellin hormone. Variety 704 with high viability showed more enzymatic activity than variety 260. The farm research was carried out at research fields of agriculture faculty of Shahrood University of Technology in 2015. This experiment was carried out in a format of factorial test and in a randomized complete block design with 3 replications. The investigated factors included: corn varieties in two levels (704 and 260), early aging in three levels (without aging, 10 days early aging and 15 days early aging) and hormone priming in three levels (zero,150 µmol cytokinin hormone and 150 µmol gibberellin hormone). The results revealed that the seed deterioration can lead to the decrease of leaf area index, chlorophyll index, relative water content, the number of rows in corn, the number of seeds in each row, the weight of 1000 seeds and harvesting index and the priming of seeds by cytokinin and gibberellin had a marked effect on the studied traits. The results uncovered that the priming of seeds by hormones before their cultivation in the fields have positive effects on quantitative and qualitative traits of the corn plant's growth.
Keywords:
#seed deterioration #hormone priming #enzyme catalase #enzyme peroxidase and corn Link
Keeping place: Central Library of Shahrood University
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