Abstarct: Regarding to the harmful effect of saturated fatty acids on human health, a great demand for a cheap and reliable source of polyunsaturated fatty acid seems to attract more attention of the producer. Appropriate source of the arachidonic acid is mortierella alpine. In this research, the effect of the various substrates including whey, glucose as carbon resources and nano magnesium particle on lipid and biomass production by mortierella alpine was investigated. Magnesium nano-particles caused the mortierella growth to induce while subsequently raised deterioration of the biomass compared to the free Magnesium nano-particles substrate. It indicated that the biomass decreased by 30%. Like biomass production and deterioration after that in nano particle media, this component induced lipid production subsequently after reaching the maximum content of lipid in biomass it decrease dramatically after reaching to the tipping point. Lipid in biomass at the media content whey substrate was accumulated 15% lipid in dry biomass after 2 fermentation days, The lipid content reached to the stationery phase, regarding to the biomass production with constant amount of lipid content it could be said the organism produced lipid in more biomass content in this circumstances the lipid content seemed to reached to constant content while it produced more than past. Magnesium nano-particles in whey media induced lipid production, in this condition lipid reached to maximum content in the shorter time than whey media without nanoparticles. It reached to the 20% of biomass after 2 days. The deterioration step of lipid induced after that which it reached to the maximum content that was 8% of biomass. it seemed this reduction provide energy for hindering of the biomass reduction. Nanoparticle in this media caused more linoleic acid production while had deterred effect on arachidonic acid production. Accumulation of oleic in lipid indicated that nanoparticle repressed the unsaturated fatty acid enzyme involved in arachidonic acid production that caused to get the results.