Abstarct: Several factors control karst development including climatology, geology and tectonic of land terrains. Assessment of karstification mainly is baxsed on the personal judgment and observations. Recently, researchers have made efforts to find a logical and scientific method to asses it numerically. New methods are classified into two main groups. First group is baxsed on the surface evaluation of solution features such as frequency and density of sinkholes and so on. Second group is baxsed on the study of hydrograph and chemograph of karstic springs. The objective of this study is to compare results of two mentioned groups in catchment area of Cheshmeh-ali Spring in Damghan. The study area lies in 32kilometrs in north of Damghan City. The Geographical Information System, Remote Sensing, and filed measurements of fractures density and frequency in several stations have been used to determine surface karst development. Monthly spring discharge rate and physicochemical parameters of spring, in 2003-2004 were used to determine the subsurface karstification rate. Furthermore, monthly measurements of discharge rate and chemical parameter were carried out in 2010-2011. GIS study results were verified by field studies. The results show that the karst development rate is average in most parts of the spring catchment area. The spring hydrograph interpretation indicate the pseudo-diffuse flow system in the region. The spring hydrograph response to rainfall shows the impact of one or more conduits in water level fluctuation zone of aquifer. The evidence is rapid response of spring to high rate of rainfall. The chemograph and springs hydrograph confirm the karstification of aquifer. Finally, it has been concluded that although both methods are able to demonstrate of the karstification process in an aquifer, it is not logical to discard the hydrograph and chemograph analysis.