Abstarct: In this study, laxyered silicate (AMP) was prepared using magnesium chloride.6H2o and 3-(tri-methoxysilyl) propylamine (MSPA) in ethanol. The surface primary amine-functionalities of AMP was subsequently converted into Schiff baxse ligand-type environments by reaction with salicylaldehyde. In continue, complexes of vanadium, molybdenum and silver salts were added to the functionalized laxyered silicate and the heterogeneous catalysts of (AMP/Sal/Ag), (AMP/Sal/Mo) and (AMP/Sal/V) were prepared. The catalysts were characterized by the Fourier transform-infrared (FT-IR), X‐ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM) along with Energy Dispersive X-ray (EDX) Spectroscopy, inductively coupled plasma (ICP), and surface area (BET) techniques. (AMP/Sal/Mo) and (AMP/Sal/V) catalysts were applied in epoxidation of cis-cyclooctene and other alkenes. The catalytic procedures with all catalysts were optimized for different parameters such as solvent, oxidant and time of reaction. It was observed the synthesized heterogeneous catalysts exhibited excellent activity in the epoxidation of alkenes. Furthermore, the as-synthesized (AMP/Sal/Ag) was employed as an efficient catalyst for the catalytic reduction of ecotoxic 4-nitrophenol 2-nitroaniline and 4-nitroaniline. The results demonstrated that the (AMP/Sal/Ag) could be a promising Nano catalyst for the catalytic reduction of 4-NP. At the end, the biological activity of (AMP / Sal / Ag), (AMP / Sal / Mo) and (AMP / Sal / V) compounds against a gram-negative bacterium (Escherichia coli) and a gram-positive bacterium (Staphylococcus aureus) were investigated. baxsed on the results, all three compounds showed high potential for inhibition of microorganism’s growth.