Abstarct: Niosome is a novel drug delivery systems. Are non-ionic vesicles are microscopic structure of two laxyers. In this study, silibinin nano¬niosomal drug formulation, by reverse-phase evaporation method of mixing the drug with a cholesterol, silibinin, polyethylenglycol2000 and Span20 certain proportions were prepared.The diameter of ¬niosomal silibinin drug nanoparticles, drug loading and encapsulation percent being respectively 192/3nm, 22% and 99%. The pattern of drug delivery vesicles using over 37 hours of dialysis were studied. The results showed that the drug release from the formulation takes place slowly. Investigate the stability of the drug formulation showed that vesicles containing medications for at least one month during the study, and was quite stable. And Drug Silibinin to form nanoniosomal vesicles remains. The formulations in the treatment of breast cancer cell line T47D was used. Drug toxicity of niosomal silibinin by MTT assay was calculated. The amount of drug IC50 niosomal silibinin after 48h, 203/5μg/ml, indicating it is less effective.